In a few exceptional cases they are detected in a high proportion of the F1 generation. Transposition events are transmitted to the F1 generation with a minimal frequency of 0.3%. Reintegration of Ac was confirmed by Southern blot analysis. About 50% of the regenerating Ac transformants showed evidence for excision of the element. Using this approach, the transpositional activity of AC was initially studied in primary transformants. The use of a chimaeric construct, where the Ac element is located in the leader of the neomycin phosphotransferase (NPT II) gene, enabled the excision of Ac to be monitored by assaying for the reconstitution of NPT II gene activity. The autonomous mobile element Activator from Zea mays was introduced into Arabidopsis thaliana via Agrobacterium-mediated gene transfer.
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